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96.96 qpcr dynamicarray microfluidic chips  (fluidigm)


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    fluidigm 96.96 qpcr dynamicarray microfluidic chips
    A. Microscopy image of the peritoneal cells after loading them into the Fluidigm C1 fluidics chip. Bright field and fluorescence microscopy images obtained after cell loading reveals the size (small vs. large) and source (RFP + LT-HSC or RFP − host) of the SPM and LPM. B. Histograms depicting the size distribution of the two main cell clusters identified by hierarchical clustering of transcription factor expression profiles, shown in D. C. t-SNE visualization of the similarity of single isolated peritoneal macrophages derived from transplanted LT-HSC (RFP + ) or host (RFP − ), based on transcription factor expression profiles. The lack of defined clusters in t-SNE map indicate that LT-HSC-derived macrophages are similar to their host-derived counterpart. D. Hierarchical clustering of transcription factor expression profiles, determined by Fluidigm Biomark single-cell multiplexed <t>qPCR,</t> of single-sorted peritoneal macrophages derived from transplanted LT-HSC (indicated with red bars) or host cells. Analysis yielded two main cell clusters that were identified as LPM (Cluster 1) and SPM (Cluster 2), based on their size distributions shown in B. E. Analysis of in vivo phagocytosis of pHrodo-labeled E. coli particles by LPM (I-A/I-E) and SPM (I-A/I-E + ) derived from transplanted LT-HSC (RFP + ) or host cells (RFP), 2 hr after i.p. injection. F. Quantification of in vivo phagocytosis of E. coli particles by LPM and SPM derived from transplanted LT-HSC (RFP + , red) or host cells (RFP, gray), as percentage of cells that contained phagocytosed E. coli among the specific cell type. G. Example of morphology, F4/80 expression and E. coli uptake by LPM and SPM. Data shown (E,F) are representative of 2 independent experiments and are mean + SD of 5 total mice (2 control and 3 chimeric animals).
    96.96 Qpcr Dynamicarray Microfluidic Chips, supplied by fluidigm, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/96%2E96+qpcr+dynamicarray+microfluidic+chips/96+96+qpcr+dynamicarray+microfluidic+chips/bio_rxiv__2021__04__08__439077-70-13-7
    Average 90 stars, based on 1 article reviews
    96.96 qpcr dynamicarray microfluidic chips - by Bioz Stars, 2026-08
    90/100 stars

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    1) Product Images from "Hematopoietic stem cell requirement for macrophage regeneration is tissue-specific"

    Article Title: Hematopoietic stem cell requirement for macrophage regeneration is tissue-specific

    Journal: bioRxiv

    doi: 10.1101/2021.04.08.439077

    A. Microscopy image of the peritoneal cells after loading them into the Fluidigm C1 fluidics chip. Bright field and fluorescence microscopy images obtained after cell loading reveals the size (small vs. large) and source (RFP + LT-HSC or RFP − host) of the SPM and LPM. B. Histograms depicting the size distribution of the two main cell clusters identified by hierarchical clustering of transcription factor expression profiles, shown in D. C. t-SNE visualization of the similarity of single isolated peritoneal macrophages derived from transplanted LT-HSC (RFP + ) or host (RFP − ), based on transcription factor expression profiles. The lack of defined clusters in t-SNE map indicate that LT-HSC-derived macrophages are similar to their host-derived counterpart. D. Hierarchical clustering of transcription factor expression profiles, determined by Fluidigm Biomark single-cell multiplexed qPCR, of single-sorted peritoneal macrophages derived from transplanted LT-HSC (indicated with red bars) or host cells. Analysis yielded two main cell clusters that were identified as LPM (Cluster 1) and SPM (Cluster 2), based on their size distributions shown in B. E. Analysis of in vivo phagocytosis of pHrodo-labeled E. coli particles by LPM (I-A/I-E) and SPM (I-A/I-E + ) derived from transplanted LT-HSC (RFP + ) or host cells (RFP), 2 hr after i.p. injection. F. Quantification of in vivo phagocytosis of E. coli particles by LPM and SPM derived from transplanted LT-HSC (RFP + , red) or host cells (RFP, gray), as percentage of cells that contained phagocytosed E. coli among the specific cell type. G. Example of morphology, F4/80 expression and E. coli uptake by LPM and SPM. Data shown (E,F) are representative of 2 independent experiments and are mean + SD of 5 total mice (2 control and 3 chimeric animals).
    Figure Legend Snippet: A. Microscopy image of the peritoneal cells after loading them into the Fluidigm C1 fluidics chip. Bright field and fluorescence microscopy images obtained after cell loading reveals the size (small vs. large) and source (RFP + LT-HSC or RFP − host) of the SPM and LPM. B. Histograms depicting the size distribution of the two main cell clusters identified by hierarchical clustering of transcription factor expression profiles, shown in D. C. t-SNE visualization of the similarity of single isolated peritoneal macrophages derived from transplanted LT-HSC (RFP + ) or host (RFP − ), based on transcription factor expression profiles. The lack of defined clusters in t-SNE map indicate that LT-HSC-derived macrophages are similar to their host-derived counterpart. D. Hierarchical clustering of transcription factor expression profiles, determined by Fluidigm Biomark single-cell multiplexed qPCR, of single-sorted peritoneal macrophages derived from transplanted LT-HSC (indicated with red bars) or host cells. Analysis yielded two main cell clusters that were identified as LPM (Cluster 1) and SPM (Cluster 2), based on their size distributions shown in B. E. Analysis of in vivo phagocytosis of pHrodo-labeled E. coli particles by LPM (I-A/I-E) and SPM (I-A/I-E + ) derived from transplanted LT-HSC (RFP + ) or host cells (RFP), 2 hr after i.p. injection. F. Quantification of in vivo phagocytosis of E. coli particles by LPM and SPM derived from transplanted LT-HSC (RFP + , red) or host cells (RFP, gray), as percentage of cells that contained phagocytosed E. coli among the specific cell type. G. Example of morphology, F4/80 expression and E. coli uptake by LPM and SPM. Data shown (E,F) are representative of 2 independent experiments and are mean + SD of 5 total mice (2 control and 3 chimeric animals).

    Techniques Used: Microscopy, Fluorescence, Expressing, Isolation, Derivative Assay, In Vivo, Labeling, Injection, Control



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    96.96 qpcr dynamicarray microfluidic chips  (fluidigm)
    90
    fluidigm 96.96 qpcr dynamicarray microfluidic chips
    A. Microscopy image of the peritoneal cells after loading them into the Fluidigm C1 fluidics chip. Bright field and fluorescence microscopy images obtained after cell loading reveals the size (small vs. large) and source (RFP + LT-HSC or RFP − host) of the SPM and LPM. B. Histograms depicting the size distribution of the two main cell clusters identified by hierarchical clustering of transcription factor expression profiles, shown in D. C. t-SNE visualization of the similarity of single isolated peritoneal macrophages derived from transplanted LT-HSC (RFP + ) or host (RFP − ), based on transcription factor expression profiles. The lack of defined clusters in t-SNE map indicate that LT-HSC-derived macrophages are similar to their host-derived counterpart. D. Hierarchical clustering of transcription factor expression profiles, determined by Fluidigm Biomark single-cell multiplexed <t>qPCR,</t> of single-sorted peritoneal macrophages derived from transplanted LT-HSC (indicated with red bars) or host cells. Analysis yielded two main cell clusters that were identified as LPM (Cluster 1) and SPM (Cluster 2), based on their size distributions shown in B. E. Analysis of in vivo phagocytosis of pHrodo-labeled E. coli particles by LPM (I-A/I-E) and SPM (I-A/I-E + ) derived from transplanted LT-HSC (RFP + ) or host cells (RFP), 2 hr after i.p. injection. F. Quantification of in vivo phagocytosis of E. coli particles by LPM and SPM derived from transplanted LT-HSC (RFP + , red) or host cells (RFP, gray), as percentage of cells that contained phagocytosed E. coli among the specific cell type. G. Example of morphology, F4/80 expression and E. coli uptake by LPM and SPM. Data shown (E,F) are representative of 2 independent experiments and are mean + SD of 5 total mice (2 control and 3 chimeric animals).
    96.96 Qpcr Dynamicarray Microfluidic Chips, supplied by fluidigm, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/96%2E96+qpcr+dynamicarray+microfluidic+chips/96+96+qpcr+dynamicarray+microfluidic+chips/bio_rxiv__2021__04__08__439077-70-13-7
    Average 90 stars, based on 1 article reviews
    96.96 qpcr dynamicarray microfluidic chips - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A. Microscopy image of the peritoneal cells after loading them into the Fluidigm C1 fluidics chip. Bright field and fluorescence microscopy images obtained after cell loading reveals the size (small vs. large) and source (RFP + LT-HSC or RFP − host) of the SPM and LPM. B. Histograms depicting the size distribution of the two main cell clusters identified by hierarchical clustering of transcription factor expression profiles, shown in D. C. t-SNE visualization of the similarity of single isolated peritoneal macrophages derived from transplanted LT-HSC (RFP + ) or host (RFP − ), based on transcription factor expression profiles. The lack of defined clusters in t-SNE map indicate that LT-HSC-derived macrophages are similar to their host-derived counterpart. D. Hierarchical clustering of transcription factor expression profiles, determined by Fluidigm Biomark single-cell multiplexed qPCR, of single-sorted peritoneal macrophages derived from transplanted LT-HSC (indicated with red bars) or host cells. Analysis yielded two main cell clusters that were identified as LPM (Cluster 1) and SPM (Cluster 2), based on their size distributions shown in B. E. Analysis of in vivo phagocytosis of pHrodo-labeled E. coli particles by LPM (I-A/I-E) and SPM (I-A/I-E + ) derived from transplanted LT-HSC (RFP + ) or host cells (RFP), 2 hr after i.p. injection. F. Quantification of in vivo phagocytosis of E. coli particles by LPM and SPM derived from transplanted LT-HSC (RFP + , red) or host cells (RFP, gray), as percentage of cells that contained phagocytosed E. coli among the specific cell type. G. Example of morphology, F4/80 expression and E. coli uptake by LPM and SPM. Data shown (E,F) are representative of 2 independent experiments and are mean + SD of 5 total mice (2 control and 3 chimeric animals).

    Journal: bioRxiv

    Article Title: Hematopoietic stem cell requirement for macrophage regeneration is tissue-specific

    doi: 10.1101/2021.04.08.439077

    Figure Lengend Snippet: A. Microscopy image of the peritoneal cells after loading them into the Fluidigm C1 fluidics chip. Bright field and fluorescence microscopy images obtained after cell loading reveals the size (small vs. large) and source (RFP + LT-HSC or RFP − host) of the SPM and LPM. B. Histograms depicting the size distribution of the two main cell clusters identified by hierarchical clustering of transcription factor expression profiles, shown in D. C. t-SNE visualization of the similarity of single isolated peritoneal macrophages derived from transplanted LT-HSC (RFP + ) or host (RFP − ), based on transcription factor expression profiles. The lack of defined clusters in t-SNE map indicate that LT-HSC-derived macrophages are similar to their host-derived counterpart. D. Hierarchical clustering of transcription factor expression profiles, determined by Fluidigm Biomark single-cell multiplexed qPCR, of single-sorted peritoneal macrophages derived from transplanted LT-HSC (indicated with red bars) or host cells. Analysis yielded two main cell clusters that were identified as LPM (Cluster 1) and SPM (Cluster 2), based on their size distributions shown in B. E. Analysis of in vivo phagocytosis of pHrodo-labeled E. coli particles by LPM (I-A/I-E) and SPM (I-A/I-E + ) derived from transplanted LT-HSC (RFP + ) or host cells (RFP), 2 hr after i.p. injection. F. Quantification of in vivo phagocytosis of E. coli particles by LPM and SPM derived from transplanted LT-HSC (RFP + , red) or host cells (RFP, gray), as percentage of cells that contained phagocytosed E. coli among the specific cell type. G. Example of morphology, F4/80 expression and E. coli uptake by LPM and SPM. Data shown (E,F) are representative of 2 independent experiments and are mean + SD of 5 total mice (2 control and 3 chimeric animals).

    Article Snippet: Single-cell multiplexed qPCR experiments were performed using Fluidigm’s (San Francisco, CA, USA) 96.96 qPCR DynamicArray microfluidic chips as previously described ( ).

    Techniques: Microscopy, Fluorescence, Expressing, Isolation, Derivative Assay, In Vivo, Labeling, Injection, Control